Speakers


Presenter
Yoshihiro ITO

Current position: Chief Scientist & Director

Institution /Faculty /Department : Cluster for Pioneering Research, RIKEN


Bio

Yoshihiro Ito was born in Gifu, Japan, in 1959. He received his Bachelor’s (1981) and Master’s (1983) degrees in polymer chemistry at Kyoto University and was awarded a doctorate in engineering from the same university in 1987. Since then he has held some posts including an assistant (1988) and associate (1996) professor at Kyoto University, research fellow at the University of California, Irvine (1992–1993), and professor of the University of Tokushima (1999). Now, he is Chief Scientist and Director of the Nano Medical Engineering Laboratory at the RIKEN Cluster for Pioneering research (from 2004). He is also visiting professors of Tokyo Institute of Technology, Tokyo Metropolitan University, Hokkaido University, Waseda University and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. His research focuses on biomaterial science, regenerative medical engineering, combinatorial bioengineering for the creation of functional polymers, and soft nanotechnology.

Clinical Application of Microarray Biochip Using Photo-Reactive Polymers

Abstract

Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 Japan

We developed a photoimmobilized allergen microarray for an allergen-specific IgE assay. First, an aqueous solution of a photoreactive poly(ethylene glycol)-based polymer was spin-coated on a plate, and an aqueous solution of each allergen was microspotted on the coated plate and allowed to dry in air. Finally, the plate was irradiated with an ultraviolet lamp for covalent immobilization. An automated machine using these plates was also developed for the assay. After a patient's blood from the finger was added to the microarray plate, the adsorbed IgE was detected by an enzyme-conjugated anti-IgE-antibody through the chemical luminescence intensity. The results were highly correlated with those by the conventional method. 41 different allergen-specific IgEs were assayed using 20 microlitter of whole blood from the finger within 30 minutes. The system is now commercially available from Nippon Chemiphar with approvement of Japanese medical insurance.

Research Specialty
Nano Medical Engineering

Recent Highlight Projects

Institute

Cluster for Pioneering Research, RIKEN